Disclosed are techniques for handling of radio frequency (RF) front-end group delays for roundtrip time (RTT) estimation. In an aspect, a network node transmits first and second RTT measurement (RTTM) signals to a user equipment (UE) and receives first and second RTT response (RTTR) signals from the UE. The network node measures the transmission times of the RTTM signals and the reception times of the RTTR signals, and the UE measures the transmission times the RTTM signals and the transmission times of the RTTR signals. The group delays of the transmit/receive chains of the network node and the UE are determined for one set of transmit/receive chains based on the first RTTM signal and first RTTR signal. The group delays of the transmit/receive chain used for the second RTTM signal and the second RTTR signal are determined relative to the group delay of the one set of transmit/receive chains.