The present disclosure directs to a system and method for automated positioning of a subject. The method may include obtaining a scout image of a subject when the subject is positioned at a first position in an apparatus. The method may also include determining location information of a reference structure of the subject based on the scout image. The method may also include determining an offset between the first position of the subject relative to a characteristic point of the apparatus according to the location information of the reference structure. The method may further include moving the subject to a second position based on the offset. The method may still further include performing, using the apparatus, a procedure relating to a target structure of the subject located at the second position.