A instrument string stretching assembly for stretching instrument strings during the manufacturing process includes a curved panel that has a plurality of channels each extending through the curved panel. A plurality of instrument strings can be inserted into a respective one of the channels having each of the instrument strings extending outwardly through the respective channel. A stretching unit is aligned with each of the plurality of channels in the curved panel and the stretching unit is actuatable into a lifting condition or a lowering condition. A clamping unit is attached to the stretching unit to grip each of the plurality of instrument strings. The stretching unit lifts the clamping unit upwardly thereby stretching the instrument strings along the plurality of channels in the curved panel.