Invention Grant
- Patent Title: Streptavidin muteins and methods of using them
-
Application No.: US17092037Application Date: 2020-11-06
-
Publication No.: US11667679B2Publication Date: 2023-06-06
- Inventor: Thomas Schmidt
- Applicant: IBA LIFESCIENCES GMBH
- Applicant Address: DE Goettingen
- Assignee: IBA LIFESCIENCES GMBH
- Current Assignee: IBA LIFESCIENCES GMBH
- Current Assignee Address: DE Goettingen
- Agency: Acuity Law Group, PC
- Agent Michael A. Whittaker
- Priority: EP 181697 2013.08.26
- Main IPC: C07K14/36
- IPC: C07K14/36 ; C12N15/62 ; G01N33/68 ; C07K1/22
Abstract:
The invention concerns novel streptavidin muteins. In one embodiment such a the mutein
(a) contains at least two cysteine residues in the region of the amino acid positions 44 to 53 with reference to the amino acid sequence of wild type streptavidin as set forth at SEQ ID NO: 212 and
(b) has a higher binding affinity than
(i) a streptavidin mutein “1” (SEQ ID NO: 112) that comprises the amino acid sequence Val44-Thr45-Ala46-Arg47 (SEQ ID NO: 98), or
(ii) wild type-streptavidin of which amino acid residues 14 to 139 are shown as SEQ ID NO: 212
for peptide ligands comprising the amino acid sequence Trp-Ser-His-Pro-Gln-Phe-Glu-Lys (SEQ ID NO: 100).
(a) contains at least two cysteine residues in the region of the amino acid positions 44 to 53 with reference to the amino acid sequence of wild type streptavidin as set forth at SEQ ID NO: 212 and
(b) has a higher binding affinity than
(i) a streptavidin mutein “1” (SEQ ID NO: 112) that comprises the amino acid sequence Val44-Thr45-Ala46-Arg47 (SEQ ID NO: 98), or
(ii) wild type-streptavidin of which amino acid residues 14 to 139 are shown as SEQ ID NO: 212
for peptide ligands comprising the amino acid sequence Trp-Ser-His-Pro-Gln-Phe-Glu-Lys (SEQ ID NO: 100).
Public/Granted literature
- US20210139545A1 STREPTAVIDIN MUTEINS AND METHODS OF USING THEM Public/Granted day:2021-05-13
Information query
