Invention Grant
- Patent Title: Split intein mediated protein polymerization for microbial production of materials
-
Application No.: US16713643Application Date: 2019-12-13
-
Publication No.: US11667682B2Publication Date: 2023-06-06
- Inventor: Fuzhong Zhang , Christopher H. Bowen , Bin Dai
- Applicant: Fuzhong Zhang , Christopher H. Bowen , Bin Dai
- Applicant Address: US MO St. Louis
- Assignee: Washington University
- Current Assignee: Washington University
- Current Assignee Address: US MO St. Louis
- Agency: Armstrong Teasdale LLP
- Main IPC: C07K14/00
- IPC: C07K14/00 ; C07K14/435 ; D01D5/06 ; D01D1/02 ; B29C48/05 ; D01F4/02 ; C12N1/20 ; C12N15/70
Abstract:
The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.
Information query
