A rolling diaphragm 5 of a rolling diaphragm pump 1 has a movable portion 31 reciprocatable together with a piston 3, a fixed portion 32 fixed to a housing 2, and a thin film portion 33 connecting the movable portion 31 and the fixed portion 32 and bending due to reciprocation of the piston 3. A rubber layer 6 is overlaid on the thin film portion 33 without being adhered to the thin film portion 33, an end portion 6a on the movable portion 31 side of the rubber layer 6 is fixed to the piston 3, and an end portion 6b on the fixed portion 32 side of the rubber layer 6 is fixed to the housing 2.