Methods, systems, and media for automated compliance determination of content items are provided. In some embodiments, the method comprises: receiving, at a server from a user device associated with a user, a request to provide a branded content item on a media content platform; in response to receiving the request, generating a transcript of a speech portion of the branded content item; generating a plurality of candidate word sequences based on the transcript; selecting a candidate word sequence from the plurality of candidate word sequences based on a similarity that is determined by comparing each of the plurality of candidate word sequences with each of a plurality of target word sequences; in response to selecting the candidate word sequence, applying a model to the selected candidate word sequence to determine whether the selected candidate word sequence contains a first disclosure statement in accordance with one or more disclosure requirements and applying the model to a content description associated with the branded content item to determine whether the content description contains a second disclosure statement in accordance with the one or more disclosure requirements; and associating the branded content item with a compliance indicator that indicates the branded content item is compliant with the one or more disclosure requirements in response to the model indicating that the selected candidate word sequence contains the first disclosure statement and in response to the model indicating that the content description contains the second disclosure statement.