The present application discloses a display substrate. The display substrate may include a base substrate; a plurality of first electrodes arranged in an array on the base substrate; and a pixel defining layer defining a plurality of openings on the base substrate. The plurality of openings may overlap the plurality of first electrodes respectively. The pixel defining layer may include a plurality of first pixel defining units and a plurality of second pixel defining units; and the plurality of first pixel defining units may be separated from one another.