A refrigerator appliance includes a cabinet with at least one food storage chamber defined in the cabinet. The refrigerator appliance also includes a sealed cooling system in fluid communication with the at least one food storage chamber via a multi-flow system. A plurality of independently cooled zones are defined within the at least one food storage chamber. The refrigerator appliance also includes a temperature control module selectively positionable in one of the plurality of zones. Methods of operating the refrigerator appliance may include and/or a controller of the refrigerator appliance may be configured for locating the temperature control module, receiving a temperature setting from the temperature control module, and adjusting operation of at least one of the sealed cooling system and the multi-flow system in response to the received temperature setting from the temperature control module.