A robotic surgical system includes an eye gaze sensing system in conjunction with a visual display of a camera image from a surgical work site. Detected gaze of a surgeon towards the display is used as input to the system. This input may be used by the system to assign an instrument to a control input device (when the user is prompted to look at the instrument), or it may be used as input to a computer vision algorithm to aid in object differentiation and seeding information, facilitating identification/differentiation of instruments, anatomical features or regions.