A septum holder having a body. The body has a disk shaped annular upper body part and a lower body part, at least one resilient elongated arm terminating with a distal enlarged element attached to the side of the body, and a septum fitted into the lower body part so that it extends downward parallel to the at least one arm. The septum is made of a single piece of cylindrically shaped resilient material. The upper part of the septum has a diameter larger than the middle part of the septum in order to form a flange that rests on an annular ledge created around the inside of the bottom section of the body when the middle part of septum slides through the open center at the bottom of the bottom section. The lowest part of the septum has a diameter that matches that of a septum in a fluid transfer component. The septum has a cavity created in its middle part into which an insert having at least one bore that functions as the seat of a needle valve is inserted.