A substrate includes a base, a pixel defining layer, a plurality of electrode pairs, and a plurality of light-emitting devices. The pixel defining layer is disposed on the base, and includes a plurality of through holes. At least one electrode pair includes a first electrode and a second electrode that are disposed at least on a hole wall of one of the through holes and at least partially opposite to each other, and the first electrode and the second electrode are insulated from each other. One of the plurality of light-emitting devices includes a liquid functional layer disposed in the through hole. The liquid functional layer is in direct contact with the first electrode and the second electrode. The liquid functional layer includes a liquid light-emitting layer configured to emit light.