A display substrate and a display device are provided. A sub-pixel in the display substrate includes a light emitting element and a pixel circuit; the light emitting element includes a first electrode, a light emitting layer and a second electrode; the pixel circuit includes a driving transistor and a storage capacitor. The sub-pixel includes a first color sub-pixel pair which includes a first pixel block and a second pixel block. In a same first color sub-pixel pair, an overlapping situation, of orthographic projections of the second electrode of one first pixel block and a gate electrode of the driving transistor of the one first pixel block on the base substrate, is the same as an overlapping situation, of orthographic projections of the second electrode of one second pixel block and a gate electrode of the driving transistor of the one second pixel block on the base substrate.