The present disclosure provides a display substrate, a manufacturing method and a display device. The display substrate comprises a scan driving circuit and a display area, wherein the scan driving circuit comprises a plurality of shift register units, a first voltage signal line, a second voltage signal line, a first clock signal line, and a second clock signal line, all of which extend in a first direction; and the display area comprises at least one driving transistor configured to drive a light-emitting element for display. At least one of the plurality of shift register units comprises an output circuit and a signal output line, wherein the output circuit is coupled to the first voltage signal line, the second voltage signal line, and the signal output line, and the signal output line extends in a second direction intersecting the first direction. The output circuit comprises a transistor that is provided between the first voltage signal line and the second voltage signal line.