Invention Grant
- Patent Title: Enzymes for trimming of glycoproteins
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Application No.: US17113975Application Date: 2020-12-07
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Publication No.: US11713453B2Publication Date: 2023-08-01
- Inventor: Remon Van Geel , Maria Antonia Wijdeven , Inge Catharina Josephina Hurkmans , Floris Louis Van Delft , Sander Sebastiaan Van Berkel
- Applicant: Synaffix B.V.
- Applicant Address: NL Oss
- Assignee: SYNAFFIX B.V.
- Current Assignee: SYNAFFIX B.V.
- Current Assignee Address: NL Oss
- Agency: Foley & Lardner LLP
- Agent Sunit Talapatra
- Priority: EP 154712 2016.02.08 EP 154739 2016.02.08 EP 173595 2016.06.08 EP 173599 2016.06.08 EP 206867 2016.12.23
- Main IPC: C12N9/24
- IPC: C12N9/24 ; A61K47/54 ; C07K1/13 ; C07K16/18 ; C12N15/52 ; C12N15/62 ; C12N15/66 ; C12P21/00

Abstract:
The invention concerns fusion proteins, wherein two endoglycosidases are fused, possibly via a linker. The fusion enzymes according to the invention have structure (1): EndoX-(L)p-EndoY (1), wherein EndoX is an endoglycosidase, EndoY is an endoglycosidase distinct from EndoX, L is a linker and p is 0 or 1. Such fusion enzymes capable of trimming glycoproteins comprising at least two distinct glycoforms in a single step. The invention further concerns the use of the fusion enzyme according to the invention for trimming glycoproteins. In another aspect, the invention relates to the process of production of the fusion enzyme. In a further aspect, the inventions concerns a process for trimming glycoproteins, comprising trimming the glycoprotein with a fusion enzyme according to the invention, to obtain a trimmed glycoprotein.
Public/Granted literature
- US20210332342A1 ENZYMES FOR TRIMMING OF GLYCOPROTEINS Public/Granted day:2021-10-28
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