An optical fiber includes: a central core portion; an intermediate layer; a trench layer; and a cladding portion. Further, Δ1>Δ2>Δ3 and 0>Δ3 are satisfied, where Δ1 is a relative refractive-index difference of the central core portion, Δ2 is a relative refractive-index difference of the intermediate layer, and Δ3 is a relative refractive-index difference of the trench layer with respect to the cladding portion, respectively, and (c−b) is smaller than 4.5 μm when Δ1 is equal to or larger than 0.36% and equal to or smaller than 0.40%, Δ2 is equal to or larger than −0.05% and equal to or smaller than 0.05%, |Δ3| is equal to or smaller than 0.25%, Δ1×|Δ3| is equal to or smaller than 0.08%2, an inner diameter of the trench layer is 2b, and an outer diameter of the trench layer is 2c.