An anti-spoofing satellite navigation and positioning method includes: receiving a positioning satellite radio frequency (RF) signal by a satellite RF receiving module, and detecting whether a power strength of the received signal exceeds a preset threshold; preprocessing the received signal by a satellite RF signal identification module, and intercepting an identifiable positioning satellite signal for identification to distinguish a real signal and a false signal; calculating the received signal to acquire real position and time information when the received signal is identified as the real signal; and sending an alarm message when the received signal is identified as the false signal. An anti-spoofing satellite navigation and positioning chip is further provided. By identifying whether the signal is a real signal or a false signal, the authenticity of the upper-layer position calculation is ensured, and the purpose of timely and accurate detection and effective resistance to spoofing attacks is achieved.