A detection method of a three-dimensional touch module includes: step S1, providing an input signal to a transmitting electrode layer; step S2, outputting a first output signal and transmitting the first output signal to a control module by a two-dimensional inputting assembly, and outputting a second output signal and transmitting the second output signal to the control module by a pressure sensing assembly; and step S3, determining, by the control module, a touch position according to the first output signal and a pressure value according to the second output signal. A three-dimensional touch module includes: a cover plate; a two-dimensional inputting assembly disposed under the cover plate and configured to output a first output signal; a pressure sensing assembly disposed under the cover plate and configured to output a second output signal; and a transmitting electrode layer disposed between the two-dimensional inputting assembly and the pressure sensing assembly.