The present disclosure relates to an organic light-emitting display substrate and a display device. The organic light-emitting display substrate includes a plurality of rows of sub-pixels, each of which includes first sub-pixels, second sub-pixels and third sub-pixels repeatedly arranged, and two adjacent rows of sub-pixels are arranged in a staggered manner, in every two adjacent rows of sub-pixels: a first sub-pixel in one row of sub-pixels and a second sub-pixel and a third sub-pixel that are adjacent to the first sub-pixel in the other row of sub-pixels, form a pixel unit, and white light brightness centers of the pixel units in a same row are located on a same straight line.