Invention Grant
- Patent Title: Identification and antibiotic characterization of pathogens in metagenomic sample
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Application No.: US16342017Application Date: 2017-10-12
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Publication No.: US11749381B2Publication Date: 2023-09-05
- Inventor: Pierre Mahe , Maud Tournoud , Stéphane Schicklin , Ghislaine Guigon , Etienne Ruppe
- Applicant: BIOMÉRIEUX
- Applicant Address: FR Marcy l'Etoile
- Assignee: BIOMÉRIEUX
- Current Assignee: BIOMÉRIEUX
- Current Assignee Address: FR Marcy l'Etoile
- Agency: Oliff PLC
- Priority: EP 193621 2016.10.13
- International Application: PCT/EP2017/076029 2017.10.12
- International Announcement: WO2018/069430A 2018.04.19
- Date entered country: 2019-04-15
- Main IPC: G16B40/20
- IPC: G16B40/20 ; C12Q1/689 ; G16B20/00 ; G16B30/00 ; C12Q1/6888

Abstract:
A method for identifying a pathogen contained in a metagenomic sample and for identifying pathogenic markers in the genome of the pathogen includes: processing the sample to extract DNA from pathogens, sequencing the extracted DNA, thereby producing a set of reads, comparing the reads to a database of genomes of known pathogens to assign reads to the pathogens; producing a pool of reads and assembling them to produce contigs, comparing the contigs to a second database of markers to check whether they contain a marker. The method further includes the step of comparing the reads to the second database to assign reads to the markers, a read being assigned to a marker if it falls entirely into or is astride the marker, and the pool also includes the reads assigned to the markers, the contigs thereby being assembled from reads assigned to a pathogen and reads assigned to markers.
Public/Granted literature
- US20190252042A1 INDENTIFICATION AND ANTIBIOTIC CHARACTERIZATION OF PATHOGENS IN METAGENOMIC SAMPLE Public/Granted day:2019-08-15
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