A micro-energy acquisition device is provided. The radio frequency circuit generates a ground voltage according to the micro-energy voltage and output the ground voltage through the ground terminal; the first unidirectional conduction component makes the ground voltage to be unidirectionally conductive to generate the first voltage; the microprocessor generates the supply voltage according to the first voltage and is operated according to the supply voltage; the first input/output port of the microprocessor is connected with the first unidirectional conduction component; since the microprocessor and the radio frequency circuit are connected in series, an electrical energy consumption speed of the micro-energy voltage is slower, the capability of data transmission is improved and an error rate of data transmission is reduced.