Invention Grant
- Patent Title: Synthesis of reversible nucleotide terminators by in-situ thio-alkyl group transfer for use in DNA sequencing by synthesis
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Application No.: US17574610Application Date: 2022-01-13
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Publication No.: US11767339B2Publication Date: 2023-09-26
- Inventor: Mong Sano Marma , Michel Perbost , Sameh Soliman , Xiao-Pei Guan
- Applicant: Miltenyi Biotec B.V. & Co. KG
- Applicant Address: DE Bergisch Gladbach
- Assignee: Miltenyi Biotech B.V. & Co. KG
- Current Assignee: Miltenyi Biotech B.V. & Co. KG
- Current Assignee Address: DE Bergisch Gladbach
- Agent Jaquelin K. Spong
- Main IPC: C07H1/00
- IPC: C07H1/00 ; C07H19/10 ; C07H19/20
Abstract:
The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7)
wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6
wherein R1, R2 and R3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B)
wherein R4, R5 R6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF4−, F−, Cl−, Br−, I−, SO42−, SO32− in aqueous solution having a pH between 3 and 7.
wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6
wherein R1, R2 and R3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B)
wherein R4, R5 R6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF4−, F−, Cl−, Br−, I−, SO42−, SO32− in aqueous solution having a pH between 3 and 7.
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