Disclosed are a coating for glass molding, a preparation method and application thereof and a mold having the coating. The coating includes a nitride layer and nano precious metal particles which are dispersed in the nitride layer. A surface roughness of the coating is 2-12 nm. The preparation method of the coating includes: cleaning a substrate and targets under an inert gas; and under a mixed atmosphere of nitrogen and the inert gas, depositing, with a high-purity W target, a high-purity Cr target and a precious metal inserted Cr target, a Cr intermediate layer, a nitride layer and nano precious metal particles on a surface of the substrate. The coating has good oxidation resistance and excellent anti-adhesion property. Moreover, the coating effectively inhibits the adhesion between the glass body and the mold.