A diagnosis kit is disclosed. The diagnosis kit according to an embodiment of the present invention includes a concentration channel into which a sample containing a methylated DNA is introduced, a concentration chamber connected to the concentration channel, wherein the methylated DNA is concentrated by an ion concentration polarization (ICP) phenomenon and moves to the concentration chamber, a sensing chamber connected to the concentration chamber to allow the methylated DNA inside the concentration chamber to move, allow the methylated DNA moved from inside of the concentration chamber to be hybridized, and allow a methylated DNA binding protein to be bound to the hybridized methylated DNA, and a sensor configured to acquire a first electrochemical signal inside the sensing chamber when the methylated DNA is hybridized and acquire a second electrochemical signal inside the sensing chamber when the methylated DNA binding protein is bound.