A multilayer anode includes an anode collector, and a plurality of anode mixture layers sequentially stacked on at least one surface of the anode collector, and including natural graphite as an anode active material. A weight ratio of the natural graphite in innermost and outermost anode mixture layers is greater than a weight ratio of the natural graphite in an anode mixture layer located between the innermost and outermost anode mixture layers, in a stacking direction of the plurality of anode mixture layers. Performance of a cell may be improved and calendering-calender contamination occurring in a calendering process and an electrode stripping phenomenon may be prevented.