A honey-based rooting gel composition and method of preparing the same are disclosed. The honey-based rooting gel composition comprises honey and symbiotic fungi such as Beauveria bassiana or Trichoderma koningii which increases plant growth and health, and may also contain a plant rooting hormone such as Indole-3-butyric acid (IBA) or other plant rooting hormones that promote root initiation, or natural rooting substances such as willow bark extracts that promote root growth. The use of honey provides an unexpected synergy to protect the cuttings from pathogens through the antibacterial and anti-fungal properties of the honey and benefits both plant and fungal growth by providing growth-promoting carbohydrates (mainly fructose and glucose) for both the plant and the germinating fungal spores. Further, the honey-based rooting gel composition simultaneously induces roots in cuttings to produce new plants and infects the new plants with beneficial symbiotic fungi that improve various properties of the growing plants.