Invention Grant
- Patent Title: DNA extraction method using microwave for next generation sequencing and use thereof
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Application No.: US16830565Application Date: 2020-03-26
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Publication No.: US11781132B2Publication Date: 2023-10-10
- Inventor: Joshua Sungwoo Yang , Jaekyung Chon , Ik Jung Choi , Hyun Min Park , Jieun Park , Jeongsun Seo , Changhoon Kim , Jong Yeon Shin , Han Sol Seo , Jiwon Shin , In Hee Hwang , Seon Hye Sim , Chang Woo Cho , Kyuin Hwang , In Seon Kim , Hyung Il Lee , Jung Hyun Cho
- Applicant: MACROGEN, INC. , PSOMAGEN, INC.
- Applicant Address: KR MD Seoul
- Assignee: MACROGEN, INC.,PSOMAGEN, INC.
- Current Assignee: MACROGEN, INC.,PSOMAGEN, INC.
- Current Assignee Address: KR Seoul; US MD Rockville
- Agency: Paratus Law Group, PLLC
- Priority: KR 20190140300 2019.11.05
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12Q1/6806 ; C12Q1/6844
Abstract:
Disclosed are a method for DNA extraction in a sample for next generation sequencing (NGS) and a method of constructing a NGS library using the extracted DNA. The method for DNA extraction includes: preparing a mixture by mixing a biological sample with a buffer; applying microwaves to the mixture; and recovering DNA. The method of constructing a NGS library includes: extracting DNA according to the method for DNA extraction; amplifying a target DNA using primers; and purifying the amplified product and subjecting the purified product to library pooling.
Public/Granted literature
- US20210130812A1 DNA EXTRACTION METHOD USING MICROWAVE FOR NEXT GENERATION SEQUENCING AND USE THEREOF Public/Granted day:2021-05-06
Information query
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