Radio frequency amplifier (200) assembly with effective prevention of RF interference. The radio frequency amplifier comprises an electrically conductive housing (301) that defines an enclosed interior of the radio frequency amplifier assembly, an electrically conductive cooling plate (415) disposed inside the electrically conductive housing and having a first side and an opposite second side, the electrically conductive cooling plate being configured to divide the enclosed interior into a first enclosed region (501) and a second enclosed region (503), and a radio frequency signal processing circuit board (223) equipped with a radio frequency signal processing circuit, the radio frequency signal processing circuit board being positioned in the first enclosed region and disposed on the first side of the electrically conductive cooling plate, and a power supply module and a controller module positioned in the second enclosed region and disposed on the opposite second side of the electrically conductive cooling plate.