The application provides a calibration method, a calibration device and a multi-phase clock circuit. The method includes: gating each of multi-phase clock signals as a first primary clock signal and gating a corresponding clock signal as a first auxiliary clock signal according to a first preset rule; gating each of the multi-phase clock signals as a second primary clock signal and gating a corresponding clock signal as a second auxiliary clock signal according to a second preset rule; obtaining a time difference between each primary clock signal and its corresponding auxiliary clock signal under the first preset rule and the second preset rule; determining a delay adjustment amount of each primary clock signal according to the time difference, and obtaining a phase error between the multi-phase clock signals according to the delay adjustment amount; and obtaining a calibration amount of the multi-phase clock signals according to the phase error.