A display substrate and a display device are provided. The display substrate includes a base substrate, an anode layer and a plurality of pixel driving circuits, the plurality of pixel driving circuits includes a first conductive layer and a second conductive layer; the anode layer includes a first anode, the second conductive layer includes a first conductive portion and a second conductive portion, the second conductive portion includes a main body, a heel block, and a connection block, the heel block is located at a side of the main body close to the first anode, and an orthographic projection of the first conductive portion on the base substrate is overlapped with an orthographic projection of the first anode on the base substrate, and an orthographic projection of the second conductive portion on base substrate is overlapped with an orthographic projection of the first anode on the base substrate.