A drone and a positioning method thereof, a drone communication system and an operation method thereof are provided. The positioning method of the drone is applicable for a drone to cruise in a flight field. The positioning method includes the following steps: flying according to a destination coordinate in the flight field; sensing a plurality of base stations via a first wireless communication module to obtain a plurality of first radio wave signals of the plurality of base stations; positioning according to the plurality of first radio wave signals to determine a current coordinate of the drone; and flying toward the destination coordinate in the flight field according to the current coordinate. In this way, the drone is capable of reliably positioning to effectively perform automatic flight missions.