A display substrate includes a plurality of first pixels and a plurality of second pixels alternately arranged in a first direction and a second direction. Each of the first pixels includes a first sub-pixel and a second sub-pixel, and each of the second pixels includes a third sub-pixel and a second sub-pixel. The second sub-pixels are evenly arranged in a matrix. The first sub-pixel and the third sub-pixel are both in a polygonal shape, and are alternately arranged in the first direction and the second direction. For one first sub-pixel and one third sub-pixel adjacent to each other in the first direction, a line connecting a vertex of the one first sub-pixel closest to the one third sub-pixel with a vertex of the one third sub-pixel closest to the one first sub-pixel intersects an extension line in the first direction and an extension line in the second direction.