Invention Grant
- Patent Title: Recombinant extracellular chitinase from
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Application No.: US17478390Application Date: 2021-09-17
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Publication No.: US11807880B2Publication Date: 2023-11-07
- Inventor: Lakshmi Prasanna Gangavaramu , Madhusudana Rao Nalam , Veerabhadra Rao Arravapalli
- Applicant: Department of Biotechnology , Council of Scientific & Industrial Research
- Applicant Address: IN New Delhi
- Assignee: Council of Scientific & Industrial Research
- Current Assignee: Council of Scientific & Industrial Research
- Current Assignee Address: IN New Delhi
- Agency: DINSMORE AND SHOHL, LLP
- Priority: IN 1711031399 2017.09.05
- The original application number of the division: US16122612 2018.09.05
- Main IPC: C12N9/42
- IPC: C12N9/42 ; A01N63/50

Abstract:
The present invention discloses a recombinant modified extracellular chitinase having an amino acid sequence set forth in SEQ ID NO. 4 or SEQ ID NO. 5 prepared by substituting two tyrosine (Y) residues with histidine (H) in the native chitinase of Brevibacillus laterosporus LAK 1210. The modified chitinase represents an advancement as it has improved thermal stability, wider optimum pH range, high efficacy, improved solubility and low toxicity. The present invention also provides compositions and improved methods for producing and purifying the recombinant modified chitinase by chitin affinity chromatography and chitin adsorption affinity chromatography using shrimp shell and crab shell, for large scale production at low cost. The modified chitinase has wide range of applications including prevention, treatment or modulation of phytopathogenic infection in a plant or a plant part.
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