Provided is a group of peripheral blood gene markers for screening benign and malignant pulmonary micro-nodules, comprising: gene sequences characterized by micronodular lung carcinoma as shown in SEQ ID NOs. 1-6, wherein the gene sequences exhibit differential expression in the peripheral blood of micronodular lung carcinoma patients and non-micronodular lung carcinoma patients. In addition, also provided is the use of the above-mentioned gene markers in preparing a product for early screening micronodular lung carcinoma. The gene markers of the invention used for early screening micronodular lung carcinoma, have high sensitivity and strong specificity; besides, they take peripheral blood, which is the easiest to collect in clinic, as the test sample. Owing to the noninvasive and simple sampling mode and high inspection compliance, they are especially applicable for the ultra-early screening of lung carcinoma for large-scale population by matching with CT and other imageological examinations, and they have a broad application prospect.