A display substrate and a display device are provided. The display substrate includes: a color filter layer including a display sub-pixel and a dummy sub-pixel and a spacer including a plurality of first spacers located in the periphery region and a plurality of second spacers located in the display region, the display sub-pixel is located in a display region and includes a first display sub-pixel, a second display sub-pixel, a third display sub-pixel, and a fourth display sub-pixel; the dummy sub-pixel is located in a periphery region the dummy sub-pixel is located in the dummy region of the periphery region, the dummy sub-pixel includes a first dummy sub-pixel, a second dummy sub-pixel, a third dummy sub-pixel, and a fourth dummy sub-pixel; an orthographic projection of the first spacer on the first base substrate has no overlap with an orthographic projection of the dummy sub-pixel on the first base substrate.