A display substrate, a display panel, a display apparatus, and a display driving method are provided. The display substrate includes: a display region and a peripheral region at a periphery thereof. Gate lines, data lines and a pixel array are in the display region. The pixel array includes pixel units each coupled to a corresponding gate line and data line, and color mixing pixel columns each including multiple pixel units emitting light of different colors and including periodic structures along the column direction. The number of pixel units in each periodic structure is constant. A gate driving circuit is in the peripheral region and includes cascaded shift registers each having a cascading signal output terminal and scanning signal output terminals each coupled to a corresponding gate line. The number of scanning signal output terminals of each shift register is equal to the number of pixel units in each periodic structure.