Disclosed herein are tracking and analysis devices, systems, and methods for optimizing workflow operations using the tracking and analysis devices and a plurality of movable and fixed tracking beacons. The movable tracking beacons may be configured for secure attachment to, or incorporation within, mobile workflow assets, while the fixed tracking beacons may be placed around the workflow environment in a grid-pattern. The tracking and analysis devices, as well as the movable and fixed tracking beacons, may each comprise any combination of a: battery, a low frequency Bluetooth module, an accelerometer, a Wi-Fi module, a USB hub, and/or an RFID reader/scanner. The tracking and analysis device may track and identify a workflow asset's location within a workflow environment within a range of about 2-3 feet by receiving and comparing relative signal strength of low frequency Bluetooth signals received from the movable tracking beacons and/or the plurality of fixed tracking beacons.