A controlled access aerosolized particle enclosure for isolation of airborne contaminants includes a frame defining a patient isolation region over a patient bed. A linkage attaches the framed enclosure to a patient treatment vehicle, and a flexible elasticized barrier is suspended by the frame for enclosing the patient isolation region. The barrier is formed from deformable planer sheets of a flexible transparent material and extending adjacent to the patient treatment surface forming a draped edge around the bed or transport. A low pressure source is in fluidic engagement with the enclosure for reducing a pressure within the enclosure below that of ambient surroundings, such that the low pressure source provides a pressure for drawing the elasticized barrier against the patient treatment surface for restricting airborne particle passage from the enclosure to the ambient surroundings, but limits the negative pressure to avoid substantial deformation or collapse of the enclosure.