A display substrate has a display area, an opening area and an isolation area located between the display area and the opening area and surrounding the opening area, and the display area is disposed at least partially around the opening area. The display substrate includes a flexible base having a via hole located in the opening area. The flexible base includes an amorphous silicon layer. The amorphous silicon layer includes a first portion located in the isolation area and a second portion located in the display area, and a thickness of the first portion is greater than a thickness of the second portion.