Invention Grant
- Patent Title: PCR measuring method and measurement device
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Application No.: US16476256Application Date: 2017-11-16
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Publication No.: US11884973B2Publication Date: 2024-01-30
- Inventor: Junko Tanaka , Takahide Yokoi , Masao Kamahori , Yoshinobu Kohara
- Applicant: HITACHI, LTD.
- Applicant Address: JP Tokyo
- Assignee: Hitachi, Ltd.
- Current Assignee: Hitachi, Ltd.
- Current Assignee Address: JP Tokyo
- Agency: Procopio, Cory, Hargreaves & Savitch LLP
- Priority: JP 17000772 2017.01.05
- International Application: PCT/JP2017/041290 2017.11.16
- International Announcement: WO2018/128013A 2018.07.12
- Date entered country: 2019-07-05
- Main IPC: C12Q1/686
- IPC: C12Q1/686 ; C12Q1/6848
Abstract:
The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.
Public/Granted literature
- US20190352699A1 PCR MEASURING METHOD AND MEASUREMENT DEVICE Public/Granted day:2019-11-21
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