A method for manufacturing a multilayer treadmill walking board assembly includes preparing a plurality of components, mixing and stirring the components to form a composite material, heating the composite material to a temperature of 140-160° C., cooling the composite material to a temperature of 80-85° C. so that the composite material is disposed at a thick state, applying the composite material on a top face of a support board, to form a coating on the support board, solidifying the coating during a time interval of 2-5 minutes, rapidly cooling the coating to a temperature of 20-30° C., with the composite material producing a stepwise multilayer deposit, thereby serially forming the wear-resistant layer, the lubricating layer, and the antistatic layer from bottom to top, and solidifying the coating to form a product.