A method for evaluating cold tolerance of Hevea brasiliensis includes: (1) taking different one-year-old germplasm plants of the Hevea brasiliensis with second whorls of leaves entering a stable period as materials, firstly culturing the materials at a normal temperature, then treating the materials at a low temperature, and finally respectively measuring relative electrical conductivities of the materials cultured at the normal temperature and physiological indexes of the cold tolerance of the materials treated at the low temperature, where a variety 93114 is used as a cold tolerance control and a Reyan 73397 is used as a sensitive control; and (2) according to changes of the physiological indexes of the cold tolerance in the germplasm plants of the Hevea brasiliensis, comprehensively evaluating the cold tolerance of the materials by using a fuzzy membership function method. When comprehensive indexes of the cold tolerance are larger, the cold tolerance of the materials is better.