A color calibration system for color correction in an optical device using a database of target colors includes a physical target card. The physical target card includes at least three unique colored patches arranged in a predetermined pattern on the physical target card, identifying indicia, and alignment indicia. The color calibration system also includes an executable machine-readable software. The software is configured to read and assign a value to the colored patches. The software is further configured to read the identifying indicia and identify the physical target card, and to read the alignment indicia for identifying individual colored patches. The software is further configured to compare color values in the optical device to known factory color values, generate a target card profile, and convert native color space for use in subsequent images under the same lighting conditions.