A display substrate comprises: an AA and an AA′. The display substrate comprises: a base, and a VSS located in the AA′, a Gate, an Init1 and a plurality of display units located in the AA, disposed on the base. The display unite comprises: a driving structure layer and a light-emitting structure layer. The light-emitting structure layer comprises: a light-emitting element. The driving structure layer comprises a pixel driving circuit configured to drive the light-emitting element to emit light. The pixel driving circuit comprises: a reset sub-circuit. The reset sub-circuit is separately connected to the Gate, the Init1, and a first pole of the light-emitting element, and is configured to provide a signal of the Init1 to the first pole of the light-emitting element for initializing the first pole. A second pole of the light-emitting element is connected to the VSS. The Inti1 is electrically connected to the VSS.