The present disclosure provides a display substrate and a method for manufacturing the same and a display device. The display substrate includes: an array of sub-pixels, a signal line and a first retaining wall structure. A first conductive pattern of the signal line is between a base substrate and a second conductive pattern of the signal line. An orthographic projection of a second portion of the first retaining wall structure on the base substrate partially overlaps an orthographic projection of the first conductive pattern on the base substrate; a boundary of the orthographic projection of the second portion on the base substrate distal to the array of sub-pixels, is between the array of sub-pixels and a boundary of the orthographic projection of the first conductive pattern on the base substrate distal to the array of sub-pixels. The orthographic projection of the second portion on the base substrate partially overlaps the orthographic projection of the second conductive pattern on the base substrate; a boundary of the orthographic projection of the second conductive pattern on the base substrate close to the array of sub-pixels, is between the array of sub-pixels and a boundary of the orthographic projection of the second portion on the base substrate, close to the array of sub-pixels.