A three-dimensional (3D) bioprinter includes: a case, a printing chamber surrounded by wall surfaces, a moving unit including a horizontal moving unit installed in a space under a bottom surface of the printing chamber and a vertical moving unit installed outside a side surface of the printing chamber, a bed disposed above a bottom surface opening of the bottom surface of the printing chamber, a first bellows which covers a space between the bed and an inner circumferential surface of the bottom surface opening, a first print module provided in the printing chamber and installed to be vertically movable by the vertical moving unit in a Z-axis direction, a second print module provided at one side of the first print module in the printing chamber and installed to be vertically movable by the vertical moving unit in the Z-axis direction, and a controller.