The present disclosure provides an array substrate and a manufacturing method thereof, and a display panel. The array substrate includes at least one marking member in at least one marking sub-region and at least one bonding lead in at least one bonding sub-region, and the at least one marking sub-region and/or the at least one bonding sub-region are further provided with: a first substrate; a first reflecting layer on the first substrate; and a first light absorbing layer on a side of the first reflecting layer distal to the first substrate. The at least one marking member and/or the at least one bonding lead is on a side of the first light absorbing layer distal to the first substrate.