A display substrate includes: a base substrate; a plurality of sub-pixels, each sub-pixel including a light-emission device including a first electrode; a plurality of signal lines including at least a first signal line and a second signal line; a signal line lead in a non-display area; and a signal line transfer structure on the base substrate, wherein the signal line transfer structure is configured to connect the signal line lead and the second signal line, the signal line transfer structure and the first signal line are located in the same layer, and the signal line transfer structure is spaced apart from the first signal line. An orthographic projection of the first electrode of at least one of the sub-pixels on the base substrate at least partially overlaps with an orthographic projection of each of the first signal line and the signal line transfer structure on the base substrate.