Laser-induced cell transfer and sorting
Abstract:
A method for transferring objects includes providing a medium in a reservoir, the medium containing objects; providing a substrate having an acceptor surface, the acceptor surface facing an opening of the reservoir; determining a first target focus point in the medium; and generating a first laser pulse or first laser pulse train focused onto the first target focus point. Pulse intensity of the first laser pulse or pulse train at the first target focus point and/or pulse duration of the first laser pulse or pulse train is/are chosen to generate a droplet of the medium ejected towards the acceptor surface. A center wavelength of the first laser pulse or pulse train is larger than 500 nm, larger than 650 nm, or between 0.9 μm and 1.35 μm. The first target focus point is determined relative to the position of at least one object of the objects.
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