Provided is a production method for a freshness-keeping bag capable of reducing the inflow of oxygen from the outside of the bag to a very low level, and thereby keeping the freshness of a content thereof for a long period of time. The present invention provides a method for producing a freshness-keeping bag formed of a film, wherein the freshness-keeping bag has a band-shaped joining region in which two ends of the film are overlapped and joined together, the joining region comprising a plurality of joined parts in which the two ends of the film are joined together, and a non-joined part which is located between adjacent ones of the joined parts and in which the overlapped ends of the film are not joined together, the non-joined part composing a non-linear ventilation passage communicating between an internal space of the bag and the outside of the bag, the method comprising the step of forming the joined parts by ultrasonic welding, wherein the step of forming the joined parts includes continuously forming the joined parts in the film, while rotating a disk-shaped ultrasonic welding anvil with respect to the film, wherein the ultrasonic welding anvil has a plurality of protrusions formed on an outer periphery thereof continuously at given intervals and correspondingly to the configuration of the joined parts of the bag.